# Series 1B- Two stressor effects on Tp-1335-follow-up experiments # Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) # of Thalassiosira pseudonana CCMP 1335 conducted to supplement series 1A experiments. # PI: U. Passow (UCSB) # version: 2018-09-04 # ========================= expt temp light_level date_expt ------------------------- 1 13.5 high 2017-05-08 ========================= light day avg_OD680 avg_OD720 F0 cell_counts QY Chla_repl_1 Chla_repl_2 POC_repl_1 POC_repl_2 PON_repl_1 PON_repl_2 ------------------------- 140 0 0.013 0.012 nd 100035 nd nd nd nd nd nd nd 140 1 0.003 0.003 655 186667 0.75 nd nd nd nd nd nd 140 2 0.025 0.011 1706 591111 0.74 nd nd nd nd nd nd 140 3 0.073 0.030 4399 1387500 0.75 nd nd nd nd nd nd 140 4 0.136 0.063 7393 1605000 0.73 nd nd nd nd nd nd 170 0 0 0 nd 100035 nd nd nd nd nd nd nd 170 1 0 0 648 230556 0.75 nd nd nd nd nd nd 170 2 0.023 0.006 1932 691667 0.75 nd nd nd nd nd nd 170 3 0.088 0.035 5190 1532500 0.75 nd nd nd nd nd nd 170 4 0.148 0.065 7986 1717500 0.73 nd nd nd nd nd nd 200 0 0.012 0.009 nd 100035 nd nd nd nd nd nd nd 200 1 0.005 0.003 720 246111 0.74 nd nd nd nd nd nd 200 2 0.034 0.016 2167 765000 0.75 nd nd nd nd nd nd 200 3 0.107 0.055 5605 1727500 0.74 nd nd nd nd nd nd 200 4 0.136 0.068 8117 1335000 0.72 nd nd nd nd nd nd 230 0 0.003 0.002 nd 100035 nd nd nd nd nd nd nd 230 1 0.008 0.003 655 229444 0.76 nd nd nd nd nd nd 230 2 0.032 0.007 2114 818333 0.77 nd nd nd nd nd nd 230 3 0.115 0.042 5592 1692500 0.75 nd nd nd nd nd nd 230 4 0.161 0.069 7387 1115000 0.71 nd nd nd nd nd nd 260 0 0.000 0.000 nd 100035 nd nd nd nd nd nd nd 260 1 0.007 0.005 674 253889 0.77 nd nd nd nd nd nd 260 2 0.047 0.028 2328 872500 0.77 nd nd nd nd nd nd 260 3 0.134 0.074 6090 1672500 0.74 nd nd nd nd nd nd 260 4 0.178 0.104 7233 1645000 0.72 nd nd nd nd nd nd 290 0 0.010 0.009 nd 100035 nd nd nd nd nd nd nd 290 1 0.083 0.076 683 267222 0.76 nd nd nd nd nd nd 290 2 0.068 0.037 2110 931667 0.76 nd nd nd nd nd nd 290 3 0.121 0.074 5212 1652500 0.74 nd nd nd nd nd nd 290 4 0.150 0.091 6218 1470000 0.71 nd nd nd nd nd nd 320 0 0.029 0.027 nd 100035 nd nd nd nd nd nd nd 320 1 0.035 0.031 702 291667 0.77 nd nd nd nd nd nd 320 2 0.073 0.049 1988 887500 0.77 nd nd nd nd nd nd 320 3 0.152 0.089 5710 1737500 0.74 nd nd nd nd nd nd 320 4 0.197 0.121 6959 1695000 0.72 nd nd nd nd nd nd 350 0 0.008 0.002 nd 100035 nd nd nd nd nd nd nd 350 1 0.018 0.011 613 184444 0.69 nd nd nd nd nd nd 350 2 0.051 0.024 1835 794167 0.76 nd nd nd nd nd nd 350 3 0.125 0.054 4810 1665000 0.76 nd nd nd nd nd nd 350 4 0.164 0.073 5608 1485000 0.69 nd nd nd nd nd nd ========================= expt temp light_level date_expt ------------------------- 2 26 high 2017-05-15 ========================= light day avg_OD680 avg_OD720 F0 cell_counts QY Chla_repl_1 Chla_repl_2 POC_repl_1 POC_repl_2 PON_repl_1 PON_repl_2 ------------------------- 140 0 0 0 nd 100067 nd nd nd nd nd nd nd 140 1 0.047 0.040 655 286000 0.77 nd nd nd nd nd nd 140 2 0.077 0.041 1706 1018333 0.76 nd nd nd nd nd nd 140 3 0.175 0.084 4399 1545000 0.72 nd nd 23158 nd 3227 nd 140 4 0.228 0.112 7393 1900000 0.71 915.9 823.2 30060 29665 4355 4276 170 0 0.015 0.013 nd 100067 nd nd nd nd nd nd nd 170 1 0.063 0.052 1079 325000 0.75 nd nd nd nd nd nd 170 2 0.083 0.053 3340 1017500 0.75 nd nd nd nd nd nd 170 3 0.170 0.093 8204 1255000 0.72 nd nd 24014 nd 3752 nd 170 4 0.210 0.117 9902 2030000 0.68 801.9 646.1 24522 22350 3463 3306 200 0 0.010 0.003 nd 100067 nd nd nd nd nd nd nd 200 1 0.024 0.016 1064 368000 0.77 nd nd nd nd nd nd 200 2 0.078 0.048 2849 772500 0.75 nd nd nd nd nd nd 200 3 0.150 0.080 7437 1540000 0.72 nd nd 17169 nd 2492 nd 200 4 0.189 0.102 9426 1710000 0.67 677.8 536.7 20526 20460 2991 3043 230 0 0.022 0.028 nd 100067 nd nd nd nd nd nd nd 230 1 0.037 0.035 nd 310000 0.75 nd nd nd nd nd nd 230 2 0.084 0.055 nd 755000 0.74 nd nd nd nd nd nd 230 3 0.180 0.094 nd 1412500 0.73 nd nd 19839 nd 2728 nd 230 4 0.218 0.111 nd 1930000 0.7 689.8 621.6 22340 22425 3437 3384 260 0 0.017 0.017 nd 100067 nd nd nd nd nd nd nd 260 1 0.031 0.023 nd 364000 0.75 nd nd nd nd nd nd 260 2 0.085 0.053 nd 1015000 0.75 nd nd nd nd nd nd 260 3 0.189 0.106 nd 1497500 0.72 nd nd 19567 nd 3148 nd 260 4 0.271 0.159 nd 2165000 0.7 727.3 830.5 31734 30248 4810 4595 290 0 0.018 0.018 nd 100067 nd nd nd nd nd nd nd 290 1 0.038 0.031 nd 320000 0.77 nd nd nd nd nd nd 290 2 0.074 0.046 nd 1002500 0.74 nd nd nd nd nd nd 290 3 0.175 0.100 nd 1550000 0.73 nd nd 16774 nd 2440 nd 290 4 0.219 0.125 nd 1805000 0.69 622.7 761.4 22705 21727 3225 3278 320 0 0.003 0.005 nd 100067 nd nd nd nd nd nd nd 320 1 0.024 0.017 nd 311000 0.75 nd nd nd nd nd nd 320 2 0.074 0.045 nd 877500 0.74 nd nd nd nd nd nd 320 3 0.186 0.101 nd 1560000 0.73 nd nd 19021 nd 2964 nd 320 4 0.261 0.149 nd 2115000 0.7 663.7 644.4 28903 29712 4407 4434 350 0 0.009 0.004 nd 100067 nd nd nd nd nd nd nd 350 1 0.024 0.008 nd 307000 0.74 nd nd nd nd nd nd 350 2 0.076 0.038 nd 865000 0.74 nd nd nd nd nd nd 350 3 0.177 0.080 nd 1612500 0.74 nd nd 17865 nd 2964 nd 350 4 0.255 0.117 nd 1630000 0.71 683.6 748.3 29157 28696 4514 4514 ========================= expt temp light_level date_expt ------------------------- 3 22 high 2017-06-26 ========================= light day avg_OD680 avg_OD720 F0 cell_counts QY Chla_repl_1 Chla_repl_2 POC_repl_1 POC_repl_2 PON_repl_1 PON_repl_2 ------------------------- 140 0 nd nd nd 100015 nd nd nd nd nd nd nd 140 1 0.053 0.033 2090 606000 0.75 nd nd nd nd nd nd 140 2 0.115 0.059 5541 978333 0.74 227.1 nd 20189 nd 2056 nd 140 3 0.167 0.087 7486 920000 0.71 194.0 nd 22030 nd 2597 nd 140 4 0.197 0.110 8567 958333 0.7 287.7 413.0 27942 27848 3835 3568 170 0 nd nd nd 100015 nd nd nd nd nd nd nd 170 1 0.051 0.019 2044 563000 0.75 nd nd nd nd nd nd 170 2 0.088 0.039 5509 1073333 0.74 152.0 nd 21598 nd 2110 nd 170 3 0.173 0.083 7875 945000 0.72 189.1 nd 27607 nd 3516 nd 170 4 0.214 0.113 9149 806667 0.71 291.3 319.7 35834 33833 5645 4367 200 0 nd nd nd 100015 nd nd nd nd nd nd nd 200 1 0.037 0.000 1910 617000 0.74 nd nd nd nd nd nd 200 2 0.117 0.035 5551 1116667 0.74 166.8 nd 20846 nd 1407 nd 200 3 0.176 0.069 7485 911667 0.7 278.5 nd 25711 nd 3030 nd 200 4 0.213 0.104 8421 880000 0.7 248.6 248.8 31850 29490 4846 3781 230 0 nd nd nd 100015 nd nd nd nd nd nd nd 230 1 0.060 0.039 1909 532000 0.75 nd nd nd nd nd nd 230 2 0.127 0.067 5471 1116667 0.74 250.6 nd 28509 nd 2651 nd 230 3 0.198 0.101 8283 1038333 0.72 290.1 nd 26049 nd 2921 nd 230 4 0.245 0.136 9237 1018333 0.71 322.3 153.1 29906 31756 4048 3515 260 0 nd nd nd 100015 nd nd nd nd nd nd nd 260 1 0.054 0.036 1868 501000 0.75 nd nd nd nd nd nd 260 2 0.121 0.073 5251 1185000 0.74 291.2 nd 18367 nd 1785 nd 260 3 0.171 0.112 7620 855000 0.71 214.3 nd 23570 nd 2326 nd 260 4 0.193 0.119 8992 1005000 0.69 121.5 207.0 28244 26791 3568 3302 290 0 nd nd nd 100015 nd nd nd nd nd nd nd 290 1 0.036 0.017 1814 521000 0.76 nd nd nd nd nd nd 290 2 0.102 0.052 5145 1031667 0.74 208.8 nd 18799 nd 1785 nd 290 3 0.155 0.084 7316 886667 0.7 338.2 nd 22255 nd 2597 nd 290 4 0.178 0.106 8382 1003333 0.68 385.7 239.0 24582 24336 3195 3036 320 0 nd nd nd 100015 nd nd nd nd nd nd nd 320 1 0.041 0.029 1885 461000 0.75 nd nd nd nd nd nd 320 2 0.100 0.054 4884 1056667 0.75 115.8 nd 15851 nd 1515 nd 320 3 0.170 0.096 7182 953333 0.72 320.8 nd 21053 nd 2651 nd 320 4 0.209 0.131 8338 906667 0.69 229.9 215.3 28584 27772 4207 3568 350 0 nd nd nd 100015 nd nd nd nd nd nd nd 350 1 0.079 0.027 1794 484000 0.75 nd nd nd nd nd nd 350 2 0.100 0.045 4788 948333 0.75 136.1 nd 16226 nd 1515 nd 350 3 0.163 0.070 7673 1108333 0.72 220.5 nd 21354 nd 2434 nd 350 4 0.197 0.092 9231 935000 0.71 467.2 186.8 26847 25507 3409 3249